验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, TF-1 cells, HL-60 cells, Raji cells |
Planning an IHC experiment? We recommend our IHCeasy WAS Ready-To-Use IHC Kit. WAS primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84714-2-RR targets WASP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag1444 Product name: Recombinant human WAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC012738 Sequence: MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Wiskott-Aldrich syndrome (eczema-thrombocytopenia) |
| 别名 | Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC012738 |
| 基因名称 | WASP |
| Gene ID (NCBI) | 7454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WASP antibody 84714-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
