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验证数据展示

  • WB analysis using 84674-4-RR

    WB analysis using 84674-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84674-4-RR (CD300A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 84674-4-RR

    IF Staining of THP-1 using 84674-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD300A antibody (84674-4-RR, Clone: 242131H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84674-4-RR

    Affinity and Kinetic Characterization of 84674-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84674-4-RR against Human CD300A were performed. The affinity constant is below 1 pM.

CD300a Recombinant monoclonal antibody

CD300a Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84674-4-RR

产品说明书

CloneNo. 242131H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84674-4-RR targets CD300a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD300a molecule
别名 CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A
计算分子量33 kDa
观测分子量40-70 kDa
GenBank蛋白编号NM_007261.4
基因名称 CD300a
Gene ID (NCBI) 11314
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UGN4-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human CD300a is a transmembrane protein, with an immunoglobulin (Ig)V-like extracellular domain and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), providing the receptor with an inhibitory capacity. The relevance of the CD300a molecule in several pathological conditions has been highlighted by multiple studies. (PMID: 37762055)

实验方案

Product Specific Protocols
IF protocol for CD300a antibody 84674-4-RRDownload protocol
WB protocol for CD300a antibody 84674-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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