验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
84643-1-RR targets IGF2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | insulin-like growth factor 2 (somatomedin A) |
| 别名 | 241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446 |
| 观测分子量 | 20 kDa |
| 基因名称 | IGF2 |
| Gene ID (NCBI) | 3481 |
| RRID | AB_3672102 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P01344 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGF2 antibody 84643-1-RR | Download protocol |
| WB protocol for IGF2 antibody 84643-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
