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验证数据展示

  • WB analysis using 84409-4-RR

    WB analysis using 84409-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84409-4-RR

    IHC staining of mouse liver using 84409-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84409-4-RR

    IHC staining of mouse liver using 84409-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 84409-4-RR

    IF Staining of mouse liver using 84409-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 84409-4-RR

    IF Staining of mouse small intestine using 84409-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84409-4-RR

    Affinity and Kinetic Characterization of 84409-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

CEACAM1/CD66a Recombinant antibody

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84409-4-RR

产品说明书

CloneNo. 241856H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse small intestine tissue
Positive IHC detected inmouse liver tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, mouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84409-4-RR targets CEACAM1/CD66a in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 CEACAM1/CD66a fusion protein Eg1423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 1
别名 241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1
计算分子量57 kDa
观测分子量120 kDa
基因名称 Ceacam1
Gene ID (NCBI) 26365
RRIDAB_3671938
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31809-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

实验方案

Product Specific Protocols
WB protocol for CEACAM1/CD66a antibody 84409-4-RRDownload protocol
IHC protocol for CEACAM1/CD66a antibody 84409-4-RRDownload protocol
IF protocol for CEACAM1/CD66a antibody 84409-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols