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验证数据展示

  • WB analysis using 84251-4-RR

    WB analysis using 84251-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84251-4-RR (COMMD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84251-4-RR

    WB analysis of HEK-293 using 84251-4-RR

    WB result of COMMD9 antibody (84251-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COMMD9 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 84251-4-RR

    Affinity and Kinetic Characterization of 84251-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84251-4-RR against Human COMMD9 were performed. The affinity constant is 1.00 nM.

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COMMD9 Recombinant monoclonal antibody

COMMD9 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84251-4-RR

产品说明书

CloneNo. 241505B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

241505B4, C11orf55, COMM domain containing 9, COMM domain-containing protein 9, HSPC166

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, A431 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84251-4-RR targets COMMD9 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35078

Product name: Recombinant human COMMD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC010892

Sequence: MAALTAEHFAALQSLLKASSKDVVRQLCQESFSSSALGLKKLLDVTCSSLSVTQEEAEELLQALHRLTRLVAFRDLSSAEAILALFPENFHQNLKNLLTKIILEHVSTWRTEAQANQISLPRLVDLDWRVDIKTSSDSISRMAVPTCLLQMKIQEDPSLCGDKPSISAVTVELSKETLDTMLDGLGRIRDQLSAVASK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 COMM domain containing 9
别名 241505B4, C11orf55, COMM domain containing 9, COMM domain-containing protein 9, HSPC166
观测分子量22 kDa
GenBank蛋白编号BC010892
基因名称 COMMD9
Gene ID (NCBI) 29099
RRIDAB_3671800
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P000
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD9, also known as HSPC166, is a member of the COMMD family of proteins. The COMMD protein family is an evolutionarily conserved gene family that plays a role in a number of critical biological processes, including inflammation, copper homeostasis, sodium balance, endosomal sorting and cancer. COMMD9 has been shown to participate in TFDP1/E2F1 activation and to play a critical role in non-small cell lung cancer (PMID: 27871936)

实验方案

Product Specific Protocols
WB protocol for COMMD9 antibody 84251-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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