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验证数据展示

  • WB analysis using 84086-1-RR

    WB analysis using 84086-1-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84086-1-RR

    Affinity and Kinetic Characterization of 84086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

CD244 Recombinant monoclonal antibody

CD244 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84086-1-RR

产品说明书

CloneNo. 241260C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84086-1-RR targets CD244 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

Fusion Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD244 molecule, natural killer cell receptor 2B4
别名 SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4
计算分子量 365 aa, 41 kDa
观测分子量70 kDa
GenBank蛋白编号BC053985
基因名称 CD244
Gene ID (NCBI) 51744
RRIDAB_3671654
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

实验方案

Product Specific Protocols
IHC protocol for CD244 antibody 84086-1-RRDownload protocol
WB protocol for CD244 antibody 84086-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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