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验证数据展示

  • WB analysis using 83798-6-RR

    WB analysis using 83798-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83798-6-RR (YAF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83798-6-RR

    FC experiment of HeLa using 83798-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug YAF2 Recombinant antibody (83798-6-RR, Clone:240834G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

YAF2 Recombinant antibody

YAF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83798-6-RR

产品说明书

CloneNo. 240834G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

YY1-associated factor 2, YY1 associated factor 2, 240834G11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Raji cells, HeLa cells, SK-N-SH cells, SH-SY5Y cells, human heart tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83798-6-RR targets YAF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 YAF2 fusion protein Ag5023 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YY1 associated factor 2
别名 YY1-associated factor 2, YY1 associated factor 2, 240834G11
计算分子量 204 aa, 23 kDa
观测分子量20 kDa
GenBank蛋白编号BC037777
基因名称 YAF2
Gene ID (NCBI) 10138
RRIDAB_3671387
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IY57
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

实验方案

Product Specific Protocols
WB protocol for YAF2 antibody 83798-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols