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验证数据展示

  • WB analysis using 83416-4-RR

    WB analysis using 83416-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83416-4-RR (Thrombomodulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 83416-4-RR

    IHC staining of human lung using 83416-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included
  • IHC staining of human lung using 83416-4-RR

    IHC staining of human lung using 83416-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included
  • IF Staining of A431 using 83416-4-RR

    IF Staining of A431 using 83416-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thrombomodulin antibody (83416-4-RR, Clone: 240365D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human placenta using 83416-4-RR

    IHC staining of human placenta using 83416-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included
  • Affinity and Kinetic Characterization of 83416-4-RR

    Affinity and Kinetic Characterization of 83416-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83416-4-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.15 nM.

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CD141/Thrombomodulin Recombinant monoclonal antibody

CD141/Thrombomodulin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83416-4-RR

产品说明书

CloneNo. 240365D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, A431 cells
Positive IHC detected inhuman lung tissue, human cervical squamous cancer tissue, human lung squamous cell carcinoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CD141/Thrombomodulin Ready-To-Use IHC Kit. CD141/Thrombomodulin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83416-4-RR targets CD141/Thrombomodulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thrombomodulin
别名 THBD, Thrombomodulin, THRM, Fetomodulin, CD141
计算分子量60 kDa
观测分子量 100-105 kDa
GenBank蛋白编号NM_000361.3
基因名称 THBD
Gene ID (NCBI) 7056
RRIDAB_3671065
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP07204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
IHC protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
IF protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols