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验证数据展示

  • IP experiment of HeLa using 83078-5-RR

    IP experiment of HeLa using 83078-5-RR

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug.

  • IF Staining of A431 using 83078-5-RR

    IF Staining of A431 using 83078-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of THP-1 using 83078-5-RR

    FC experiment of THP-1 using 83078-5-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

p300 Recombinant antibody

p300 Recombinant Antibody for IF/ICC, FC (Intra), IP, ELISA
Cat No. 83078-5-RR

产品说明书

CloneNo. 230369D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), IP, ELISA

别名

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells, HepG2 cells
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83078-5-RR targets p300 in IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 E1A binding protein p300
别名 EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
计算分子量 264 kDa
GenBank蛋白编号NM_001429
基因名称 EP300
Gene ID (NCBI) 2033
RRIDAB_3670795
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ09472
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for p300 antibody 83078-5-RRDownload protocol
IP protocol for p300 antibody 83078-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols