• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Neutralization experiment of NeutraKine® BMP-4 using 69015-1-Ig (same clone as 69015-1-PBS)

    Neutralization experiment of NeutraKine® BMP-4 using 69015-1-Ig (same clone as 69015-1-PBS)

    Recombinant human BMP-4 (Cat.NO. HZ-1045) induces alkaline phosphatase production in the ATDC-5 cell line (using pNPP as chromogenic substrate for detection) in a dose dependent manner (blue curve, bottom X-left Y). The activity of human BMP-4 (200 ng/mL HZ-1045) is neutralized by mouse anti-human BMP-4 antibody 69015-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 3-10 μg/mL. This data was developed using the same antibody clone with 69015-1-PBS in a different storage buffer formulation.

  • Sample test of MP51307-1

    Sample test of MP51307-1

    Cytometric bead array of cell line supernatant using MP51307-1, BMP-4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60897-1-PBS. Detection antibody: 69015-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: HT-29, TF-1.

  • Cytometric bead array standard curve of MP51307-1

    Cytometric bead array standard curve of MP51307-1

    Cytometric bead array standard curve of MP51307-1, BMP-4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60897-1-PBS. Detection antibody: 69015-1-PBS. Standard:HZ-1045. Range: 6.25-100 ng/mL.

NeutraKine® BMP-4 Monoclonal antibody, PBS Only (Detector)

NeutraKine® BMP-4 Monoclonal Antibody for Neutralization, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 69015-1-PBS

产品说明书

CloneNo. 3B5A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Neutralization, Cytometric bead array, Indirect ELISA, Sample test

别名

BMP 4, BMP4, BMP-4, 3B5A7, BMP2B

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy BMP4 Ready-To-Use IHC Kit. BMP4 primary antibody included.

产品信息

69015-1-PBS targets NeutraKine® BMP-4 as part of a matched antibody pair:

MP51307-1: 60897-1-PBS capture and 69015-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Neutralization, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 human Humankine BMP-4 protein HZ-1045 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bone morphogenetic protein 4
别名 BMP 4, BMP4, BMP-4, 3B5A7, BMP2B
基因名称 BMP4
Gene ID (NCBI) 652
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP12644
储存缓冲液 PBS only, pH 7.3.
储存Store at -80°C.

背景介绍

BMP4, also named as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 have been shown to be components of serum and are thought to be responsible for this differentiation. BMP4 are expressed mainly in neurons, with minimal expression detected in astrocytes and oligodendrocytes. This antibody can be used to neutralize the bioactivity of BMP-4.