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验证数据展示

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (MRPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MRPP3 Monoclonal antibody

MRPP3 Monoclonal Antibody for WB, ELISA
Cat No. 68168-1-Ig

产品说明书

CloneNo. 1G9A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Pig

应用

WB, ELISA

别名

KIAA0391, MRPP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells, pig liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68168-1-Ig targets MRPP3 in WB, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Pig
免疫原

CatNo: Ag15629

Product name: Recombinant human KIAA0391 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 230-583 aa of BC032221

Sequence: LLLLEDIKKVITPSKKNYNDCIQGALLHQDVNTAWNLYQELLGHDIVPMLETLKAFFDFGKDIKDDNYSNKLLDILSYLRNNQLYPGESFAHSIKTWFESVPGKQWKGQFTTVRKSGQCSGCGKTIESIQLSPEEYECLKGKIMRDVIDGGDQYRKTTPQELKRFENFIKSRPPFDVVIDGLNVAKMFPKVRESQLLLNVVSQLAKRNLRLLVLGRKHMLRRSSQWSRDEMEEVQKQASCFFADDISEDDPFLLYATLHSGNHCRFITRDLMRDHKACLPDAKTQRLFFKWQQGHQLAIVNRFPGSKLTFQRILSYDTVVQTTGDSWHIPYDEDLVERCSCEVPTKWLCLHQKT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0391
别名 KIAA0391, MRPP3
计算分子量 583 aa, 67 kDa
观测分子量 65-67 kDa
GenBank蛋白编号BC032221
基因名称 MRPP3
Gene ID (NCBI) 9692
RRIDAB_2935263
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15091
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

实验方案

Product Specific Protocols
WB protocol for MRPP3 antibody 68168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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