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验证数据展示

  • WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51873-2

    Cytometric bead array sample test of MP51873-2

    Cytometric bead array sample test of MP51873-2, DOHH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68151-1-PBS. Detection antibody: 68151-3-PBS.

  • WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    WB analysis of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    WB result of DOHH antibody (68151-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOHH transfected LNCaP cells. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    IF Staining of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51873-2

    Cytometric bead array standard curve of MP51873-2

    Cytometric bead array standard curve of MP51873-2, DOHH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68151-1-PBS. Detection antibody: 68151-3-PBS. Standard:Ag13041. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO Validated

DOHH Monoclonal antibody, PBS Only (Capture)

DOHH Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68151-1-PBS

产品说明书

CloneNo. 1H5H7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1H5H7, EC:1.14.99.29, hDOHH, HEAT-like repeat-containing protein 1, HLRC1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68151-1-PBS targets DOHH as part of a matched antibody pair:

MP51873-2: 68151-1-PBS capture and 68151-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13041

Product name: Recombinant human DOHH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-302 aa of BC002817

Sequence: MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine hydroxylase/monooxygenase
别名 1H5H7, EC:1.14.99.29, hDOHH, HEAT-like repeat-containing protein 1, HLRC1
计算分子量 33 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC002817
基因名称 DOHH
Gene ID (NCBI) 83475
RRIDAB_2935259
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BU89
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

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