验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, HeLa cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67836-1-Ig targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human |
| 免疫原 | OXCT1 fusion protein Ag24792 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | 3-oxoacid CoA transferase 1 |
| 别名 | 1G1B9, 3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, EC:2.8.3.5, OXCT |
| 计算分子量 | 520 aa, 56 kDa |
| 观测分子量 | 52-56 kDa |
| GenBank蛋白编号 | BC009001 |
| 基因名称 | OXCT1 |
| Gene ID (NCBI) | 5019 |
| RRID | AB_2918598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P55809 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
| IHC protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
| IF protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
| FC protocol for OXCT1 antibody 67836-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth | ||
Sci Rep Imaging phenotype reveals that disulfirams induce protein insolubility in the mitochondrial matrix | ||
Cancer Med Induction of resistance to neurotrophic tropomyosin-receptor kinase inhibitors by HMGCS2 via a mevalonate pathway |
