验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| RIP | See 1 publications below |
产品信息
66990-1-Ig targets TP73 in WB, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26793 Product name: Recombinant human TP73 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-100 aa of NM_001126240 Sequence: MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein p73 |
| 别名 | 1D12A12, P73, tumor protein p73 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_001126240 |
| 基因名称 | TP73 |
| Gene ID (NCBI) | 7161 |
| RRID | AB_2882307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O15350 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TP73 antibody 66990-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression | ||
Biochem Pharmacol Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA. | ||
Carcinogenesis Construction and identification of lncRNA/circRNA coregulated ceRNA networks in gemcitabine-resistant bladder carcinoma |
