验证数据展示
经过测试的应用
| Positive WB detected in | HSCT6 cells, T-47D cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66512-1-Ig targets ING2 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat, mouse |
| 免疫原 |
CatNo: Ag19291 Product name: Recombinant human ING2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-280 aa of BC030128 Sequence: MLGQQQQQLYSSAALLTGERSRLLTCYVQDYLECVESLPHDMQRNVSVLRELDNKYQETLKEIDDVYEKYKKEDDLNQKKRLQQLLQRALINSQELGDEKIQIVTQMLELVENRARQMELHSQCFQDPAESERASDKAKMDSSQPERSSRRPRRQRTSESRDLCHMANGIEDCDDQPPKEKKSKSAKKKKRSKAKQEREASPVEFAIDPNEPTYCLCNQVSYGEMIGCDNEQCPIEWFHFSCVSLTYKPKGKWYCPKCRGDNEKTMDKSTEKTKKDRRSR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | inhibitor of growth family, member 2 |
| 别名 | ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2 |
| 计算分子量 | 280 aa, 33 kDa |
| 观测分子量 | 33-35 kDa |
| GenBank蛋白编号 | BC030128 |
| 基因名称 | ING2 |
| Gene ID (NCBI) | 3622 |
| RRID | AB_2881875 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H160 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ING2 antibody 66512-1-Ig | Download protocol |
| FC protocol for ING2 antibody 66512-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
