验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells, rat spleen tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, Ramos cells, Daudi cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66466-1-Ig targets JAK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag17940 Product name: Recombinant human JAK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-363 aa of BC132729 Sequence: EDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEW 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Janus kinase 1 (a protein tyrosine kinase) |
| 别名 | JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1 |
| 计算分子量 | 1154 aa, 133 kDa |
| 观测分子量 | 133 kDa |
| GenBank蛋白编号 | BC132729 |
| 基因名称 | JAK1 |
| Gene ID (NCBI) | 3716 |
| RRID | AB_2881834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P23458 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for JAK1 antibody 66466-1-Ig | Download protocol |
| WB protocol for JAK1 antibody 66466-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Cannabis suppresses antitumor immunity by inhibiting JAK/STAT signaling in T cells through CNR2. | ||
Cell Mol Immunol SENP6 restricts the IFN-I-induced signaling pathway and antiviral activity by deSUMOylating USP8 | ||
Mol Neurodegener Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice. | ||
Nat Commun Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes | ||
Cardiovasc Diabetol Targeting TFAM K76 acetylation attenuates mitochondrial dysfunction and kidney injury in diabetic kidney disease. |
