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验证数据展示

  • WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50930-1

    Cytometric bead array standard curve of MP50930-1

    Cytometric bead array standard curve of MP50930-1, SRD5A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66329-1-PBS. Detection antibody: 66329-2-PBS. Standard:Ag23351. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

SRD5A1 Monoclonal antibody, PBS Only (Capture)

SRD5A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66329-1-PBS

产品说明书

CloneNo. 1D5D8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Steroid 5 alpha reductase 1, SR type 1, S5AR 1, EC:1.3.1.22, 3-oxo-5-alpha-steroid 4-dehydrogenase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66329-1-PBS targets SRD5A1 as part of a matched antibody pair:

MP50930-1: 66329-1-PBS capture and 66329-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23351

Product name: Recombinant human SRD5A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-163 aa of BC007033

Sequence: MATATGVAEERLLAALAYLQCAVGCAVFARNRQTNSVYGRHALPSHRLRVPARAAWVVQELPSLALPLYQYASESAPRLRSAPNCILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDWVTDPRFLIGFGLWLTGML

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
别名 Steroid 5 alpha reductase 1, SR type 1, S5AR 1, EC:1.3.1.22, 3-oxo-5-alpha-steroid 4-dehydrogenase 1
观测分子量 32 kDa
GenBank蛋白编号BC007033
基因名称 SRD5A1
Gene ID (NCBI) 6715
RRIDAB_2881710
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP18405
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.