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验证数据展示

  • WB analysis of human brain using 66131-1-Ig

    WB analysis of human brain using 66131-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66131-1-Ig (TRAPPC9,NIBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66131-1-Ig

    IHC staining of human kidney using 66131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66131-1-Ig (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAPPC9, NIBP Monoclonal antibody

TRAPPC9, NIBP Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66131-1-Ig

产品说明书

CloneNo. 1C4F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRAPPC9,NIBP, TRS120, Tularik gene 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66131-1-Ig targets TRAPPC9, NIBP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TRAPPC9, NIBP fusion protein Ag8949 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 trafficking protein particle complex 9
别名 IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRAPPC9,NIBP, TRS120, Tularik gene 1 protein
计算分子量 139 kDa
观测分子量 128 kDa
GenBank蛋白编号BC006206
基因名称 NIBP/Trappc9
Gene ID (NCBI) 83696
RRIDAB_2881530
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96Q05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation and play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter.

实验方案

Product Specific Protocols
WB protocol for TRAPPC9, NIBP antibody 66131-1-IgDownload protocol
IHC protocol for TRAPPC9, NIBP antibody 66131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols