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验证数据展示

  • WB analysis of mouse brain using 60152-1-Ig

    WB analysis of mouse brain using 60152-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 60152-1-Ig

    FC experiment of HeLa using 60152-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug FGF12 Monoclonal antibody (60152-1-Ig, Clone:2F11A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FGF12 Monoclonal antibody

FGF12 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 60152-1-Ig

产品说明书

CloneNo. 2F11A10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

FGF-12, 2F11A10, FGF 12, FHF1, FHF-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60152-1-Ig targets FGF12 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FGF12 fusion protein Ag5046 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor 12
别名 FGF-12, 2F11A10, FGF 12, FHF1, FHF-1
计算分子量 181 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC022524
基因名称 FGF12
Gene ID (NCBI) 2257
RRIDAB_10644325
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61328
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

实验方案

Product Specific Protocols
WB protocol for FGF12 antibody 60152-1-IgDownload protocol
FC protocol for FGF12 antibody 60152-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols