验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
17061-1-AP targets PMFBP1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10789 Product name: Recombinant human PMFBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 673-1022 aa of BC028423 Sequence: ELQCCSTQLESSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVKYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKALGPSRTESTQREKVCGTLGWKGLPQDMGQRMDLTKYIGMPHCPGTSAIGQKNKCDFFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polyamine modulated factor 1 binding protein 1 |
| 别名 | PMF 1 binding protein, PMFBP1 |
| 计算分子量 | 1022 aa, 119 kDa |
| 观测分子量 | 119 kDa |
| GenBank蛋白编号 | BC028423 |
| 基因名称 | PMFBP1 |
| Gene ID (NCBI) | 83449 |
| RRID | AB_2299881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TBY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PMFBP1 antibody 17061-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. | ||
Mol Genet Genomic Med Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects. | ||
Alzheimers Res Ther Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response. | ||
