验证数据展示

WB analysis using 16409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16409-1-AP (Biglycan antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human lung cancer using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human colon cancer using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human kidney using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human stomach cancer using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human stomach cancer using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human liver using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IHC staining of human liver using 16409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with BGN/Biglycan antibody included
IF Staining of HepG2 using 16409-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Biglycan antibody (16409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Biglycan Polyclonal antibody

Biglycan Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16409-1-AP

产品说明书

引用文献(43)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (3)

别名

BGN, DSPG1, PG S1, PGI, PG-S1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (43)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	COLO 320 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, pig cartilage tissue
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue, human kidney tissue, human liver tissue, human liver cancer tissue, human lung tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy BGN/Biglycan Ready-To-Use IHC Kit. BGN/Biglycan primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:200-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 28 publications below
IHC	See 14 publications below
IF	See 12 publications below
IP	See 1 publications below

产品信息

16409-1-AP targets Biglycan in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, Cell treatment
经测试反应性	human, mouse, rat, pig
文献引用反应性	human, mouse, rat, canine
免疫原	CatNo: Ag0683 Product name: Recombinant human Biglycan protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-368 aa of BC004244 Sequence: MWPLWRLVSLLALSQALPFEQRGFWDFTLDDGPFMMNDEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLARVPSGLPDLKLLQVVYLHSNNITKVGVNDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKK 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	biglycan
别名	BGN, DSPG1, PG S1, PGI, PG-S1
计算分子量	42 kDa
观测分子量	40-48 kDa
GenBank蛋白编号	BC004244
基因名称	Biglycan
Gene ID (NCBI)	633
RRID	AB_10804656
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P21810
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

实验方案

Product Specific Protocols
IF protocol for Biglycan antibody 16409-1-AP	Download protocol
IHC protocol for Biglycan antibody 16409-1-AP	Download protocol
WB protocol for Biglycan antibody 16409-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	J Nanobiotechnology Youthful small extracellular vesicles restore the function and reparative capacity of inflammatory-impaired periodontal ligament stem cells via delivering protein biglycan for bone regeneration Authors - Jiaqi Yang KD Validated View Article
human	WB,IHC	Theranostics Enhancer-mediated NR2F2 recruitment activates BGN to promote tumor growth and shape tumor microenvironment in papillary thyroid cancer. Authors - Mei Tao KD Validated View Article
		Adv Sci (Weinh) BGN/MDK Axis in the Melanoma Tumor Microenvironment Strengthens Tumor Malignancy by Modulating Cancer Cells and Cancer-Associated Fibroblasts Crosstalk Authors - Hao-Ze Shi View Article
rat	WB	Biomaterials Genetically engineered extracellular vesicles-based collagen-targeting bioinspired scaffold for tendon regenerative repair. Authors - Yan-Jing Zhang KD Validated View Article
mouse	WB	Cell Death Dis Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway. Authors - Qi Cui View Article
rat	WB	Acta Biomater Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1 Authors - Jinwei Lu View Article

验证数据展示

WB analysis using 16409-1-AP

IHC staining of human colon using 16409-1-AP

IHC staining of human lung cancer using 16409-1-AP

IHC staining of human colon cancer using 16409-1-AP

IHC staining of human kidney using 16409-1-AP

IHC staining of human stomach cancer using 16409-1-AP

IHC staining of human stomach cancer using 16409-1-AP

IHC staining of human liver using 16409-1-AP

IHC staining of human liver using 16409-1-AP

IF Staining of HepG2 using 16409-1-AP

Biglycan Polyclonal antibody

Biglycan Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Youthful small extracellular vesicles restore the function and reparative capacity of inflammatory-impaired periodontal ligament stem cells via delivering protein biglycan for bone regeneration

Enhancer-mediated NR2F2 recruitment activates BGN to promote tumor growth and shape tumor microenvironment in papillary thyroid cancer.

BGN/MDK Axis in the Melanoma Tumor Microenvironment Strengthens Tumor Malignancy by Modulating Cancer Cells and Cancer-Associated Fibroblasts Crosstalk

Genetically engineered extracellular vesicles-based collagen-targeting bioinspired scaffold for tendon regenerative repair.

Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.

Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1

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