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- KD/KO Validated
IFT88 Polyclonal antibody
IFT88 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine and More (4)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
338
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells, mouse thymus tissue |
Positive IP detected in | knockout cells and WT cells, HEK-293 cells |
Positive IHC detected in | human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells, hTERT-RPE1 cells, C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13967-1-AP targets IFT88 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse, rat, chicken, zebrafish, pig, canine |
Immunogen | IFT88 fusion protein Ag4980 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | intraflagellar transport 88 homolog (Chlamydomonas) |
Synonyms | IFT88, TPR repeat protein 10, TTC10 |
Calculated Molecular Weight | 94 kDa |
Observed Molecular Weight | 88-95 kDa |
GenBank Accession Number | BC030776 |
Gene Symbol | IFT88 |
Gene ID (NCBI) | 8100 |
RRID | AB_2121979 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13099 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IFT88 antibody 13967-1-AP | Download protocol |
IHC protocol for IFT88 antibody 13967-1-AP | Download protocol |
IF protocol for IFT88 antibody 13967-1-AP | Download protocol |
IP protocol for IFT88 antibody 13967-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis. | ||
Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. | ||
Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. |