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Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, FC (Intra), IP, ELISA, IF and More (1)

Conjugate

Unconjugated

Cat No : 15176-1-AP

Print datasheet

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1



经过测试的应用

Positive WB detected inHeLa cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15176-1-AP targets Gamma Tubulin in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA, IF Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen Gamma Tubulin fusion protein Ag7070 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma 1
Synonyms TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000619
Gene Symbol Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2878113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

实验方案

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IHC protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IP protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Cell

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

Authors - Shihao Zhang
humanIF,WB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
mouseIF

PLoS Biol

Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.

Authors - Suchismita Panda
mouseWB

Cell Death Differ

Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.

Authors - Zhigang Li
humanWB

Elife

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.

Authors - Laura Bozal-Basterra
humanWB,IF

J Nanobiotechnology

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Authors - Linjia Su