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Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF

Host / Isotype

Rabbit / IgG


human, mouse, rat, canine


WB, IHC, FC (Intra), IP, ELISA, IF and More (1)



Cat No : 15176-1-AP

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TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1


Positive WB detected inHeLa cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells


Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


15176-1-AP targets Gamma Tubulin in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA, IF Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen Gamma Tubulin fusion protein Ag7070 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma 1
Synonyms TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000619
Gene Symbol Gamma Tubulin
Gene ID (NCBI) 7283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.


Product Specific Protocols
WB protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IHC protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
IP protocol for Gamma Tubulin antibody 15176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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