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ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat No : 17711-1-AP
|Positive WB detected in||L02 cells, mouse brain tissue, rat liver tissue, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, hTERT-RPE1 cells, hTERT-RPE1 cells, NIH3T3 cells, mouse embryonic fibroblasts|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17711-1-AP targets ARL13B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Applications||IF, IHC, IP, WB, ELISA|
|Cited Applications||FC, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, rat, canine, chicken, monkey, pig, sheep, Xenopus, zebrafish|
|Immunogen||ARL13B fusion protein Ag12015|
|Host / Isotype||Rabbit / IgG|
|Full Name||ADP-ribosylation factor-like 13B|
|Synonyms||ARL13B, ARL2 like protein 1, ARL2L1, JBTS8|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||40-48 kDa, 66 kDa|
|GenBank accession number||BC094725|
|Gene ID (NCBI)||200894|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two speciﬁc bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
|Product Specific Protocols|
|WB protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IHC protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IF protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IP protocol for ARL13B antibody 17711-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Guided self-organization and cortical plate formation in human brain organoids.
Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.
Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.
A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.
Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.
Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution.