Recombinant Human FGFR2(IIIc) protein (hFc Tag, Myc Tag, His Tag)(HPLC verified)
种属
Human
纯度
>95 %, SDS-PAGE
>90 %, SEC-HPLC
标签
hFc Tag, Myc Tag, His Tag
生物活性
EC50: 75-300 ng/mL
验证数据展示
产品信息
| 纯度 | >95 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Immobilized Human FGF basic (His tag) at 1 μg/mL (100 μL/well) can bind Human FGFR2 (IIIc) (hFc tag, Myc tag, His tag) with a linear range of 75-300 ng/mL. |
| 来源 | HEK293-derived Human FGFR2 (IIIc) protein Arg22-Glu377 (Accession# P21802-1) with a human IgG1 Fc tag, a Myc tag and a His tag at the C-terminus. |
| 基因ID | 2263 |
| 蛋白编号 | P21802-1 |
| 预测分子量 | 68 kDa |
| SDS-PAGE | 85-100 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis. Ligand binding leads to the activation of several signaling pathway, such as RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.
参考文献:
1.Eswarakumar VP, et al. (2005). Cytokine Growth Factor Rev. 16(2):139-149. 2.Pike, KG. (2017). Topics in Medicinal Chemistry. 8. 3.Azoury SC, et al. (2017). Int J Biol Sci. 13(12):1479-1488.
