Recombinant Pig TNF-alpha protein (rFc Tag)
种属
Pig
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Pig TNF-alpha protein Leu77-Leu232 (Accession# P23563) with a rabbit IgG Fc tag at the N-terminus. |
| 基因ID | 397086 |
| 蛋白编号 | P23563 |
| 预测分子量 | 44.4 kDa |
| SDS-PAGE | 43-48 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).
参考文献:
1. Agbanoma G. et al. (2012) J Immunol. 188: 1307-17 2. Kriegler M. et al. (1988) Cell. 53: 45-53. 3. Theiss AL. et al. (2005) J Biol Chem. 280: 36099-109 4. Swardfager W. et al. (2010) Biol Psychiatry. 68:930-41 5. Locksley RM.et al. (2001) Cell. 104(4):487-501 6. provided by RefSeq, Jul 2008
