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Recombinant Mouse CD28 protein (hFc Tag)

种属

Mouse

纯度

>95 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 23-91 ng/mL

Cat no : Eg0805

Print datasheet

Synonyms

CD28 antigen, T-cell-specific surface glycoprotein CD28

验证数据展示

  • Purity of Recombinant Mouse CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD28 (hFc tag) with a linear range of 23-91 ng/mL.

    Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD28 (hFc tag) with a linear range of 23-91 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD28 (hFc tag) with a linear range of 23-91 ng/mL.
来源 HEK293-derived Mouse CD28 protein Asn20-Leu150 (Accession# P31041) with a Human IgG1 Fc tag at the C-terminus.
基因ID 12487
蛋白编号 P31041
预测分子量 41.5 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway. CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death.

参考文献:

1.W Lesslauer, et al. (1986) Eur J Immunol. 16(10):1289-96.
2.C H June, et al. (1990) Immunol Today. 11(6):211-6.
3.T M Williams, et al. (1992) J Immunol. 148(8):2609-16.
4.P S Linsley, et al. (1993) Annu Rev Immunol. 11:191-212.
5.R J Peach, et al. (1994) J Exp Med. 180(6):2049-58.
6.Edward J Evans, et al. (2005) Nat Immunol. 6(3):271-9.
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