Recombinant Human PDI protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human PDI protein Asp18-Leu508 (Accession# P07237) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 5034 |
| 蛋白编号 | P07237 |
| 预测分子量 | 81.3 kDa |
| SDS-PAGE | 80-90 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
P4HB (Prolyl 4-hydroxylase subunit beta) belongs to the disulfide bond isomerase family and is a highly abundant multifunctional enzyme responsible for hydroxylating prolyl residues in procollagen. It can catalyze the formation, breakage and rearrangement of disulfide bonds through two thioredoxin structural domains, which are important for maintaining the oxidative environment in the endoplasmic reticulum. P4HB dysregulation has been reported in neoplasms, diabetes mellitus, neurodegenerative diseases and cardiovascular disorders and its up-regulation in most of the tumors correlates with a poor prognosis, thus P4HB may serve as a potent target for a variety of cancers. (PMID: 38029391) P4HB serves as a novel target for UFMylation, and its UFMylation deficiency promotes P4HB protein degradation via the ubiquitin-proteasome pathway, which leads to impairment of mitochondrial function and exacerbates oxidative stress and ERS; Therefore, P4HB UFMylation may be a therapeutic target for liver mitochondrial function-related diseases. (PMID: 35753586)
参考文献:
1. Feng D, et al. Aging Dis. 2023;15(6):2369-2380. 2. Gao X, et al. J Extracell Vesicles. 2021;10(5):e12060. 3. Zhu J, et al. Free Radic Biol Med. 2022;188:277-286. 4. Wu Y, et al. Oncol Lett. 2021;21(2):95.
