Recombinant Human IL-12 (linked heterodimer) protein (hFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
hFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human IL-12B Ile23-Ser328 (Accession# P29460) +linker+IL-12A Arg23-Ser219 (Accession# P29459) with a human IgG1 Fc tag at the C-terminus. |
| 基因ID | 3593(IL-12B),3592(IL-12A) |
| 蛋白编号 | P29460 (IL-12B), P29459 (IL-12A) |
| 预测分子量 | 85.3 kDa |
| SDS-PAGE | 85-100 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine that induces the production of interferon-gamma (IFN-gamma), favours the differentiation of T helper 1 (T(H)1) cells and forms a link between innate resistance and adaptive immunity. It consists of two subunits, p35 and p40, which are encoded by IL12A and IL12B, respectively. Furthermore, IL-12 binds to the IL-12 receptor, which is a heterodimeric receptor formed by the IL-12R-β1 and IL-12R-β2 subunits.
参考文献:
1.Trinchieri, Giorgio. (2003) Nat Rev Immunol.3(2):133-146. 2.Picard, Capucine et al. (2002) Am J Hum Genet.70(2):336-348. 3.Lin, Eugene et al. (2019) Front Neurol.10:1065.
