Recombinant Human EGFR protein (hFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
hFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human EGFR protein Leu25-Ser645 (Accession# P00533-1) with a human IgG1 Fc tag at the C-terminus. |
| 基因ID | 1956 |
| 蛋白编号 | P00533-1 |
| 预测分子量 | 94.6 kDa |
| SDS-PAGE | 100-130 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTKs) and exerts critical functions in epithelial cell physiology. EGFR is a receptor of tyrosine kinase (RTK). It is consisted of a C-terminus intracellular region that possesses the kinase activity, and an N-terminus extracellular ligand-binding site, a hydrophobic transmembrane domain. EGFR is frequently mutated and/or overexpressed in different types of human cancers and is the target of multiple cancer therapies currently adopted in the clinical practice. Mutations in this gene are associated with lung cancer.
参考文献:
1. Schlessinger J. et al. (2014). Cold Spring Harb Perspect Biol. 6(3). 2. Yoshida T. et al. (2010). Biochem Pharmacol. 80(5):613-23. 3. Wilson KJ. et al. (2012). Growth Factors. 30(2):107-16. 4. Sigismund S. et al. (2018). Mol Oncol. 12(1):3-20.
