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Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified)

种属

Human

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg3377

Print datasheet

Synonyms

CGA, HCG, CG-alpha, FSH alpha, FSHA

验证数据展示

  • Purity of Recombinant Human hCG Alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human hCG Alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human hCG Alpha was greater than 90% as determined by SEC-HPLC.

    The purity of Human hCG Alpha was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human hCG Alpha protein Ala25-Ser116 (Accession# P01215) with a rabbit IgG Fc tag at the C-terminus.
基因ID 1081
蛋白编号 P01215
预测分子量 36.5 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

The glycoprotein hormone human chorionic gonadotropin (hCG) is known for its pregnancy-supporting role. It is produced mainly by trophoblast cells and also produced by the secretory endometrium at the time of implantation. The hCG protein complex is composed of two noncovalently linked subunits, alpha (CGA) and beta (CGB). Both subunits are highly glycosylated. Its alpha subunit is common to luteinizing hormone (LH), follicle-stimulating hormone (FSH) and thyrotropin (TSH), whereas the respective beta-subunits confer the biological specificities.

参考文献:

1.Licht, P. et al. (2007) Mol.Cell.Endocrinol.269,85-92.
2.Tsampalas,M.(2010) J.Reprod.Immunol.85,93-98.
3. Hearn,M.T.(2000) J.Mol.Recognit.13,223-278.
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