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验证数据展示

  • WB analysis of HEK-293 cells using 15057-1-AP (same clone as 15057-1-PBS)

    WB analysis of HEK-293 cells using 15057-1-AP (same clone as 15057-1-PBS)

    WB result of anti-TPPP3 (15057-1-AP, 1:500) with HEK-293 cells (siRNA transfected with) by Dr. Yupeng Chen. (Negative control or TPPP3 specific siRNAs were transfected into HEK-293 cells with the final concentration of 20 nM. Forty eight hours after transfection, cells were lysed with RIPA buffer and subjected to SDS PAGE). This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 15057-1-AP (same clone as 15057-1-PBS)

    WB analysis of PC-3 using 15057-1-AP (same clone as 15057-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 15057-1-AP (same clone as 15057-1-PBS)

    IHC staining of human stomach cancer using 15057-1-AP (same clone as 15057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15057-1-AP (TPPP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TPPP3 Polyclonal antibody, PBS Only

TPPP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15057-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Tubulin polymerization-promoting protein family member 3, TPPP/p20, p25gamma, p20, CGI-38

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15057-1-PBS targets TPPP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7040

Product name: Recombinant human TPPP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC000691

Sequence: MAASTDMAGLEESFRKFAIHGDPKASGQEMNGKNWAKLCKDCKVADGKSVTGTDVDIVFSKVKGKSARVINYEEFKKALEELATKRFKGKSKEEAFDAICQLVAGKEPANVGVTKAKTGGAVDRLTDTSRYTGSHKERFDESGKGKGIAGRQDILDDSGYVSAYKNAGTYDAKVKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin polymerization-promoting protein family member 3
别名 Tubulin polymerization-promoting protein family member 3, TPPP/p20, p25gamma, p20, CGI-38
计算分子量 19 kDa
观测分子量 19-20 kDa
GenBank蛋白编号BC000691
基因名称 TPPP3
Gene ID (NCBI) 51673
RRIDAB_2878105
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPPP3 (tubulin polymerization promoting protein 3), also named as TPPP/p20, is a new member of the TPPP family which binds tubulin and induces tubulin polymerization and microtubule bundling.TPPP3 has been shown to play an important role in cell proliferation and may be implicated in tumorigenesis and metastasis (19633818). TPPP3 was also identified as specific marker for the connective tissues that envelope tendons due to its distinct expression in the developing tendon sheath (19235716). This antibody detects the 19-20 kDa TPPP3 in HeLa and PC-3 cells. The non-specific bands between 40 and 60 kDa were also occasionally observed.

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