验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25366-1-AP targets TMEM182 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag21906 Product name: Recombinant human TMEM182 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-115 aa of BC130308 Sequence: SDYWLLATEVGRCSGEKNIENVTFHHEGFFWRCWFNGIVEENDSNIWKFWYTNQPPSKNCTHAYLSPYPFMRGEHNSTSYDSAVIYRGF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 182 |
| 别名 | Transmembrane protein 182, UNQ6974/PRO21957 |
| 计算分子量 | 229 aa, 26 kDa |
| 观测分子量 | 15 kDa 26 kDa |
| GenBank蛋白编号 | BC130308 |
| 基因名称 | TMEM182 |
| Gene ID (NCBI) | 130827 |
| RRID | AB_2880046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZP80 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM182 antibody 25366-1-AP | Download protocol |
| WB protocol for TMEM182 antibody 25366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
