TMEM182 Polyclonal antibody

TMEM182 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25366-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

Transmembrane protein 182, UNQ6974/PRO21957

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25366-1-AP targets TMEM182 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 TMEM182 fusion protein Ag21906 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 182
别名 Transmembrane protein 182, UNQ6974/PRO21957
计算分子量 229 aa, 26 kDa
观测分子量 15 kDa 26 kDa
GenBank蛋白编号BC130308
基因名称 TMEM182
Gene ID (NCBI) 130827
RRIDAB_2880046
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZP80
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM182 antibody 25366-1-APDownload protocol
IHC protocol for TMEM182 antibody 25366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB Bioadv

TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression

Authors - Hirofumi Morihara
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