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验证数据展示

  • IF Staining of NCCIT using CL488-68071

    IF Staining of NCCIT using CL488-68071

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using CoraLite® Plus 488 STRA8 antibody (CL488-68071, Clone: 1D7E3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated STRA8 Monoclonal antibody

STRA8 Monoclonal Antibody for IF/ICC
Cat No. CL488-68071

产品说明书

CloneNo. 1D7E3

宿主/亚型

Mouse / IgG1

种属反应性

Human, rabbit, mouse

应用

IF/ICC

别名

STRA8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNCCIT cells
Planning an IHC experiment? We recommend our IHCeasy STRA8 Ready-To-Use IHC Kit. STRA8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68071-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68071 targets STRA8 in IF/ICC applications and shows reactivity with Human, rabbit, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rabbit, mouse
免疫原 STRA8 fusion protein Ag29680 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stimulated by retinoic acid gene 8 homolog (mouse)
别名 STRA8
计算分子量 37 kDa
观测分子量 43-60 kda
GenBank蛋白编号NM_182489
基因名称 STRA8
Gene ID (NCBI) 346673
RRIDAB_3084394
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z7C7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STRA8 antibody CL488-68071Download protocol
Standard Protocols
Click here to view our Standard Protocols