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验证数据展示

  • WB analysis using 28632-1-AP (same clone as 28632-1-PBS)

    WB analysis using 28632-1-AP (same clone as 28632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 28632-1-AP (same clone as 28632-1-PBS)

    WB analysis of HeLa using 28632-1-AP (same clone as 28632-1-PBS)

    WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells. This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC38A1 antibody included
  • IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC38A1 antibody included
  • Featured Product
  • KD/KO验证

SLC38A1 Polyclonal antibody, PBS Only

SLC38A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28632-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SLC38A1 Ready-To-Use IHC Kit. SLC38A1 primary antibody included.

产品信息

28632-1-PBS targets SLC38A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30088

Product name: Recombinant human SLC38A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-74 aa of BC010620

Sequence: MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDEYIPGTTSLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 38, member 1
别名
计算分子量 487 aa, 54 kDa
观测分子量54 kDa, 60-70 kDa
GenBank蛋白编号BC010620
基因名称 SLC38A1
Gene ID (NCBI) 81539
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2H9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.