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验证数据展示

  • WB analysis of mouse pancreas using 15855-1-AP

    WB analysis of mouse pancreas using 15855-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15855-1-AP (SLC25A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 15855-1-AP

    IP experiment of mouse heart using 15855-1-AP

    IP result of anti-SLC25A3 (IP:15855-1-AP, 4ug; Detection:15855-1-AP 1:500) with mouse heart tissue lysate 4400ug.

SLC25A3 Polyclonal antibody

SLC25A3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 15855-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, ELISA and More (1)

别名

OK/SW cl.48, PHC, phosphate carrier, Phosphate transport protein, PiC, PTP, SLC25A3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse pancreas tissue, MCF-7 cells
Positive IP detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15855-1-AP targets SLC25A3 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, RIP
经测试反应性 human, mouse
文献引用反应性mouse, pig
免疫原

CatNo: Ag8698

Product name: Recombinant human SLC25A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-265 aa of BC000998

Sequence: MFSSVAHLARANPFNTPHLQLVHDGLGDLRSSSPGPTGQPRRPRNLAAAAVEEYSCEFGSAKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSVTLKEDGVRGLAKGWAPTFLGYSMQGLCKFGFYEVFKVLYSNMLGEENTYLWRTSLYLAASASAEFFADIALAPMEAAKVRIQTQPGYANTLRDAAPKMYKEEGLKAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECSKPEQLVVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
别名 OK/SW cl.48, PHC, phosphate carrier, Phosphate transport protein, PiC, PTP, SLC25A3
计算分子量 40 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC000998
基因名称 SLC25A3
Gene ID (NCBI) 5250
RRIDAB_2190058
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A3 antibody 15855-1-APDownload protocol
IP protocol for SLC25A3 antibody 15855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

J Biol Chem

A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction.

Authors - Xiaomin Zhao
View Article
mouseWB

Am J Physiol Cell Physiol

miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart.

Authors - Baseler Walter A WA
View Article
pigWB,RIP

Vet Microbiol

circEZH2 inhibits opening of mitochondrial permeability transition pore via interacting with PiC and up-regulating RSAD2.

Authors - Jianxiong Guo
  • KD Validated
View Article