验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| RIP | See 1 publications below |
产品信息
15855-1-AP targets SLC25A3 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, pig |
| 免疫原 |
CatNo: Ag8698 Product name: Recombinant human SLC25A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-265 aa of BC000998 Sequence: MFSSVAHLARANPFNTPHLQLVHDGLGDLRSSSPGPTGQPRRPRNLAAAAVEEYSCEFGSAKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSVTLKEDGVRGLAKGWAPTFLGYSMQGLCKFGFYEVFKVLYSNMLGEENTYLWRTSLYLAASASAEFFADIALAPMEAAKVRIQTQPGYANTLRDAAPKMYKEEGLKAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECSKPEQLVVT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3 |
| 别名 | OK/SW cl.48, PHC, phosphate carrier, Phosphate transport protein, PiC, PTP, SLC25A3 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC000998 |
| 基因名称 | SLC25A3 |
| Gene ID (NCBI) | 5250 |
| RRID | AB_2190058 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00325 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for SLC25A3 antibody 15855-1-AP | Download protocol |
| WB protocol for SLC25A3 antibody 15855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction. | ||
Am J Physiol Cell Physiol miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart. | ||
Vet Microbiol circEZH2 inhibits opening of mitochondrial permeability transition pore via interacting with PiC and up-regulating RSAD2.
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