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验证数据展示

  • WB analysis using 21932-1-AP

    WB analysis using 21932-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21932-1-AP

    WB analysis using 21932-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

SLC14A2 Polyclonal antibody

SLC14A2 Polyclonal Antibody for WB, ELISA
Cat No. 21932-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

hUT A6, HUT2, SLC14A2, Urea transporter 2, Urea transporter, kidney, UT A2, UT2, UTA, UTR

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21932-1-AP targets SLC14A2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16561

Product name: Recombinant human SLC14A2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 431-610 aa of BC110445

Sequence: EANRIYYLTVKSGEEEKAPSGGGGEHPPTAGPKVEEGSEAVLSKHRSVFHIEWSSIRRRSKVFGKGEHQERQNKDPFPYRYRKPTVELLDLDTMEESSEIKVETNISKTSWIRSSMAASGKRVSKALSYITGEMKECGEGLKDKSPVFQFFDWVLRGTSQVMFVNNPLSGILIILGLFIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 14 (urea transporter), member 2
别名 hUT A6, HUT2, SLC14A2, Urea transporter 2, Urea transporter, kidney, UT A2, UT2, UTA, UTR
计算分子量 920 aa, 101 kDa
观测分子量43 kDa, 101 kDa
GenBank蛋白编号BC110445
基因名称 SLC14A2
Gene ID (NCBI) 8170
RRIDAB_3085679
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15849
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC14A2, also known as human urea transporter -2 (HUT2), is the human homolog of UT-A2 and the only kidney-specific urea transporter cloned in humans. Urea transport in the kidney is mediated by a family of transporter proteins, including renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). UT-A proteins expressed in the kidney play a critical role in generating the hypertonic medulla, which is an integral component of the urinary concentrating mechanism (PMID: 11590132, 11880324). The 4.2-kb hUT-A1 cDNA encodes a 920-amino acid peptide, which is 89% identical to the rat UT-A1 protein (PMID: 11502588).

实验方案

Product Specific Protocols
WB protocol for SLC14A2 antibody 21932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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