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验证数据展示

  • WB analysis of human plasma using 29931-1-AP

    WB analysis of human plasma using 29931-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 29931-1-AP (CBG/Corticosteroid binding globulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 29931-1-AP

    IP experiment of human placenta using 29931-1-AP

    IP result of anti-SERPINA6/CBG (IP:29931-1-AP, 4ug; Detection:29931-1-AP 1:1000) with human placenta tissue lysate 960 ug.

  • IHC staining of human kidney using 29931-1-AP

    IHC staining of human kidney using 29931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29931-1-AP (Corticosteroid binding globulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 29931-1-AP

    IHC staining of human kidney using 29931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29931-1-AP (Corticosteroid binding globulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SERPINA6/CBG Polyclonal antibody

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 29931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

CBG, Serpin A6, SERPINA6, Corticosteroid binding globulin, Corticosteroid-binding globulin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29931-1-AP targets SERPINA6/CBG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31861

Product name: Recombinant human Corticosteroid binding globulin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 268-405 aa of BC058021

Sequence: PDKGKMNTVIAALSRDTINRWSAGLTSSQVDLYIPKVTISGVYDLGDVLEEMGIADLFTNQANFSRITQDAQLKSSKVVHKAVLQLNEEGVDTAGSTGVTLNLTSKPIILRFNQPFIIMIFDHFTWSSLFLARVMNPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
别名 CBG, Serpin A6, SERPINA6, Corticosteroid binding globulin, Corticosteroid-binding globulin
计算分子量 405 aa, 45 kDa
观测分子量 45-55 kDa
GenBank蛋白编号BC058021
基因名称 SERPINA6
Gene ID (NCBI) 866
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08185
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

实验方案

Product Specific Protocols
IHC protocol for SERPINA6/CBG antibody 29931-1-APDownload protocol
IP protocol for SERPINA6/CBG antibody 29931-1-APDownload protocol
WB protocol for SERPINA6/CBG antibody 29931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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