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验证数据展示

  • WB analysis using 30257-1-AP

    WB analysis using 30257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30257-1-AP (SCML1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 30257-1-AP

    WB analysis of HepG2 using 30257-1-AP

    WB result of SCML1 antibody (30257-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML1 transfected HepG2 cells.

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  • KD/KO验证

SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, ELISA
Cat No. 30257-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

SCML1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30257-1-AP targets SCML1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag33003

Product name: Recombinant human SCML1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 95-260 aa of BC026159

Sequence: MKKNEVYETFSYPESYSPTLPVSRRENNSPSNLPRPSFCMEEYQRAELEEDPILSRTPSPVHPSDFSEHNCQPYYASDGATYGSSSGLCLGNPRADSIHNTYSTDHASAAPPSVTRSPVENDGYIEEGSITKHPSTWSVEAVVLFLKQTDPLALCPLVDLFRSHEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg-like 1 (Drosophila)
别名 SCML1
计算分子量 37 kDa
观测分子量42 kDa
GenBank蛋白编号BC026159
基因名称 SCML1
Gene ID (NCBI) 6322
RRIDAB_2935536
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for SCML1 antibody 30257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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