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CoraLite® Plus 488-conjugated RPA1 Monoclonal antibody

RPA1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G3F11

Cat No : CL488-67973

Print datasheet

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

验证数据展示

  • FC experiment of HeLa using CL488-67973

    FC experiment of HeLa using CL488-67973

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RPA1 (CL488-67973, Clone:1G3F11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67973-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67973 targets RPA1 in FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Rat
Immunogen RPA1 fusion protein Ag28239 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A1, 70kDa
Synonyms HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70
Calculated Molecular Weight 616 aa, 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC018126
Gene Symbol RPA1
Gene ID (NCBI) 6117
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27694
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RPA1 antibody CL488-67973Download protocol
Standard Protocols
Click here to view our Standard Protocols