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验证数据展示

  • IHC staining of human liver using 26084-1-AP

    IHC staining of human liver using 26084-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 26084-1-AP

    IF Staining of HepG2 using 26084-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF217 antibody (26084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 26084-1-AP

    IHC staining of human liver using 26084-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNF217 Polyclonal antibody

RNF217 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 26084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

C6orf172, IBRDC1, ring finger protein 217, RNF217

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26084-1-AP targets RNF217 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22022

Product name: Recombinant human RNF217 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-275 aa of BC026087

Sequence: MKVQLGQVEIKCPITECFEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYRQLRFFGDHTSNLSIFGCKYRYLPERPHLRRLVRGSVCAGKLFIAPLIMVLGLALGAIAVVIVEEIKTYWNLISGRTRNQTQHLAPQPVLLSDMLYCLKQVFICISYLLPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 217
别名 C6orf172, IBRDC1, ring finger protein 217, RNF217
计算分子量 275 aa, 32 kDa
观测分子量 59, 32 kDa
GenBank蛋白编号BC026087
基因名称 RNF217
Gene ID (NCBI) 154214
RRIDAB_3085839
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E3 ubiquitin-protein ligase RNF217, also known as IBR domain-containing protein 1 (IBRDC1), as a transmembrane (TM) domain-containing RBR-type E3 ubiquitin-protein ligase, belongs to a highly conserved member of the RING finger family. RNF217 mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis(Uniprot, PMID: 33895792).

实验方案

Product Specific Protocols
IF protocol for RNF217 antibody 26084-1-APDownload protocol
IHC protocol for RNF217 antibody 26084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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