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RGS8 Polyclonal antibody

RGS8 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27394-1-AP

Print datasheet

Synonyms

RGS8

验证数据展示

  • WB analysis using 27394-1-AP

    WB analysis using 27394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27394-1-AP (RGS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 27394-1-AP

    IF Staining of mouse cerebellum using 27394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 27394-1-AP

    IF Staining of mouse cerebellum using 27394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27394-1-AP targets RGS8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RGS8 fusion protein Ag26535 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling 8
Synonyms RGS8
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC069718
Gene Symbol RGS8
Gene ID (NCBI) 85397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

实验方案

Product Specific Protocols
WB protocol for RGS8 antibody 27394-1-APDownload protocol
IHC protocol for RGS8 antibody 27394-1-APDownload protocol
IF protocol for RGS8 antibody 27394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols