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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

RFX1 Monoclonal antibody, PBS Only

RFX1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68057-1-PBS

产品说明书

CloneNo. 1G10H6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, Indirect ELISA

别名

RFX, EF-C, EF C, 1G10H6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68057-1-PBS targets RFX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原

CatNo: Ag25442

Product name: Recombinant human RFX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 62-289 aa of BC049826

Sequence: QPQAQPPGGQKQYVTELPAVPAPSQPTGAPTPSPAPQQYIVVTVSEGAMRASETVSEASPGSTASQTGVPTQVVQQVQGTQQRLLVQTSVQAKPGHVSPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSPVQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQERSVVQATPQAPKPGPVQPLTVQGLQPVHVAQEVQQLQQVPVPH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulatory factor X, 1 (influences HLA class II expression)
别名 RFX, EF-C, EF C, 1G10H6
计算分子量 105 kDa
观测分子量135 kDa
GenBank蛋白编号BC049826
基因名称 RFX1
Gene ID (NCBI) 5989
RRIDAB_2918798
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP22670
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).