验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, PMA treated HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87619-1-RR targets Phospho-MSK1 (Ser376) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 kinase, 90kDa, polypeptide 5 |
| 别名 | MSK1 (phospho S376), MSK1 (Ser376), phospho MSK1, Phospho-MSK1, Phospho-MSK1(S376) |
| 计算分子量 | 549aa,62 kDa; 802aa,90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC017187 |
| 基因名称 | MSK1 |
| Gene ID (NCBI) | 9252 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75582 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitogen- and stress-activated kinase 1 (MSK1) is a protein kinase that plays a crucial role in the regulation of gene expression in response to various stimuli, including mitogens, stress, and inflammatory signals. ERK1/2 or p38 MAPK activate MSK1 by phosphorylating Thr581 and Ser360; this primes the C-terminal domain, which then autophosphorylates Ser376 (and Ser212) to unlock the N-terminal catalytic site. (PMID: 15568999)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MSK1 (Ser376) antibody 87619-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
