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验证数据展示

  • WB analysis using 86238-5-RR (same clone as 86238-5-PBS)

    WB analysis using 86238-5-RR (same clone as 86238-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86238-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 86238-5-RR (same clone as 86238-5-PBS)

    IHC staining of human tonsillitis using 86238-5-RR (same clone as 86238-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 86238-5-RR (same clone as 86238-5-PBS)

    IHC staining of human tonsillitis using 86238-5-RR (same clone as 86238-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsil using 86238-5-RR (same clone as 86238-5-PBS)

    IF Staining of human tonsil using 86238-5-RR (same clone as 86238-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsil tissue using Pan-Keratin (Type I) antibody (86238-5-RR, Clone: 251001E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsil using 86238-5-RR (same clone as 86238-5-PBS)

    IF Staining of human tonsil using 86238-5-RR (same clone as 86238-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsil tissue using Pan-Keratin (Type I) antibody (86238-5-RR, Clone: 251001E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-5-PBS in a different storage buffer formulation.

Pan-Keratin (Type I) Recombinant monoclonal antibody, PBS Only

Pan-Keratin (Type I) Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86238-5-PBS

产品说明书

CloneNo. 251001E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

Pan Keratin, keratin, pan-CK, Pan-CK (Type I), Pan-Keratin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86238-5-PBS targets Pan-Keratin (Type I) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Pan-Keratin (Type I)
别名 Pan Keratin, keratin, pan-CK, Pan-CK (Type I), Pan-Keratin
观测分子量44-50 kDa
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15323
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.

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