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验证数据展示

  • WB analysis of T-47D using 67269-1-Ig (same clone as 67269-1-PBS)

    WB analysis of T-47D using 67269-1-Ig (same clone as 67269-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 67269-1-Ig (PSG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50302-2

    Cytometric bead array standard curve of MP50302-2

    Cytometric bead array standard curve of MP50302-2, PSG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67269-1-PBS. Detection antibody: 67269-4-PBS. Standard:Ag28604. Range: 3.125-100 ng/mL.

PSG1 Monoclonal antibody, PBS Only (Capture)

PSG1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67269-1-PBS

产品说明书

CloneNo. 1C6C4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

1C6C4, B1G1, CD66 antigen-like family member F, CD66f, DHFRP2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67269-1-PBS targets PSG1 as part of a matched antibody pair:

MP50302-2: 67269-1-PBS capture and 67269-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28604

Product name: Recombinant human PSG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 35-349 aa of BC022338

Sequence: QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pregnancy specific beta-1-glycoprotein 1
别名 1C6C4, B1G1, CD66 antigen-like family member F, CD66f, DHFRP2
计算分子量 426 aa, 48 kDa
观测分子量 40-70 kDa
GenBank蛋白编号BC022338
基因名称 PSG1
Gene ID (NCBI) 5669
RRIDAB_2882539
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP11464
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).

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