PPIA-Specific Polyclonal antibody
PPIA-Specific Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | NIH3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
35001-1-AP targets PPIA-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Recombinant Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | peptidylprolyl isomerase A (cyclophilin A) |
| Synonyms | Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | NM_021130 |
| Gene Symbol | PPIA |
| Gene ID (NCBI) | 5478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62937 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPIA, also named as CYPA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. This antibody is immunized with full length gene PPIA and absorbed by PPIF. So this antibody is specific to PPIA.
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways. |