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验证数据展示

  • WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

PHYH Polyclonal antibody, PBS Only

PHYH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12858-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12858-1-PBS targets PHYH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3569

Product name: Recombinant human PHYH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC029512

Sequence: MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phytanoyl-CoA 2-hydroxylase
别名
计算分子量 39 kDa
观测分子量 36 kDa, 70 kDa
GenBank蛋白编号BC029512
基因名称 PHYH
Gene ID (NCBI) 5264
RRIDAB_2163745
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14832
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.