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验证数据展示

  • WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

PHYH Polyclonal antibody, PBS Only

PHYH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12858-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12858-1-PBS targets PHYH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHYH fusion protein Ag3569 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phytanoyl-CoA 2-hydroxylase
别名
计算分子量 39 kDa
观测分子量 36 kDa, 70 kDa
GenBank蛋白编号BC029512
基因名称 PHYH
Gene ID (NCBI) 5264
RRIDAB_2163745
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14832
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.