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验证数据展示

  • WB analysis using 87087-1-RR

    WB analysis using 87087-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87087-1-RR (OSBPL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 87087-1-RR

    IF Staining of HepG2 using 87087-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OSBPL10 antibody (87087-1-RR, Clone: 252248A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

OSBPL10 Recombinant monoclonal antibody

OSBPL10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87087-1-RR

产品说明书

CloneNo. 252248A4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

ORP 10, ORP10, ORP-10, OSBP related protein 10, OSBP9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuH-7 cells, PANC-1 cells, HepG2 cells, A431 cells, HT-29 cells, rat brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy OSBPL10 Ready-To-Use IHC Kit. OSBPL10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87087-1-RR targets OSBPL10 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag7861

Product name: Recombinant human OSBPL10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC003168

Sequence: MERAVQGTDGGGGSNSSSRSSSRATSAGSSPSCSLAGRGVSSRSAAAGLGGGGSRSSPGSVAASPSGGGGRRREPALEGVLSKYTNLLQGWQNRYFVLDFEAGILQYFVNEQSKHQKPRGVLSLSGAIVSLSDEAPHMLVVYSANGEMFKLRAADAKEKQFWVTQLRACAKYHMEMNSKSAPSSRSRSLTLLPHGTPNSASPCSQRHLSVGAPGVVTITHHKSPAAARRAKSQYSGQLHEVREMMNQVEGQQKNLVHAIESLPGSGPLTALDQDLLLLKATSAATLSCLGECLNLLQQSVHQAGQPSQKPGASENILGWHGSKSHSTEQLKNGTLGSLPSASANITWA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oxysterol binding protein-like 10
别名 ORP 10, ORP10, ORP-10, OSBP related protein 10, OSBP9
计算分子量 84 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC003168
基因名称 OSBPL10
Gene ID (NCBI) 114884
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BXB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.

实验方案

Product Specific Protocols
IF protocol for OSBPL10 antibody 87087-1-RRDownload protocol
WB protocol for OSBPL10 antibody 87087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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