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验证数据展示

  • WB analysis using 23191-1-AP

    WB analysis using 23191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 23191-1-AP

    IHC staining of human skeletal muscle using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 23191-1-AP

    IHC staining of human skeletal muscle using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 40x lens).

MMADHC Polyclonal antibody

MMADHC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23191-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C2orf25, cblD, CL25022, MMADHC, My011

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, NIH3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23191-1-AP targets MMADHC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MMADHC fusion protein Ag19343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
别名 C2orf25, cblD, CL25022, MMADHC, My011
计算分子量 296 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC022859
基因名称 MMADHC
Gene ID (NCBI) 27249
RRIDAB_2879229
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3L0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.

实验方案

Product Specific Protocols
WB protocol for MMADHC antibody 23191-1-APDownload protocol
IHC protocol for MMADHC antibody 23191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols